| 5/01/21 17:00 | UD Ibiza Eivissa | R. C. Celta de Vigo | 5:2 |
| 5/01/21 17:00 | Córdoba C. F. | Getafe C.F. | 1:0 |
| 5/01/21 19:00 | Zamora CF | Villarreal C. F. | 1:4 |
| 5/01/21 19:00 | Linares Deportivo | Sevilla F. C. | 0:2 |
| 5/01/21 21:00 | A. D. Alcorcón | Real Zaragoza | 2:1 |
| 5/01/21 21:15 | Marbella FC | Real Valladolid C. F. | 2:3t.pro. |
| 6/01/21 12:00 | CF La Nucia | Elche C. F. | 0:1 |
| 6/01/21 12:00 | Club Portugalete | Levante U. D. | 1:2 |
| 6/01/21 12:00 | C. D. Numancia de Soria | U.D. Almeria | 1:2 |
| 6/01/21 13:00 | UD Socuellamos | C. D. Leganés | 0:2 |
| 6/01/21 17:00 | CD Artistico Navalcarnero | U. D. Las Palmas | 1:0 |
| 6/01/21 17:00 | Club Haro Deportivo | Rayo Vallecano de Madrid | 1:3 |
| 6/01/21 17:00 | Cultural y Deportiva Leonesa | Granada C. F. | 1:2t.pro. |
| 6/01/21 17:00 | Union Mutilvera | Real Betis Balompié | 1:3 |
| 6/01/21 18:00 | UE Cornella | Club Atlético de Madrid | 1:0 |
| 6/01/21 19:00 | R. C. Deportivo de La Coruña | Deportivo Alavés | 0:1 |
| 6/01/21 19:00 | Olot | C. A. Osasuna | 0:3 |
| 6/01/21 19:00 | Burgos C. F. | R.C.D. Espanyol | 0:2 |
| 6/01/21 20:00 | CD Castellon | C. D. Tenerife | 0:2 |
| 6/01/21 21:00 | CF Fuenlabrada | R. C. D. Mallorca | 9:8pen. |
| 6/01/21 21:00 | Málaga C. F. | Real Oviedo | 1:0t.pro. |
| 7/01/21 19:00 | CF Pontevedra | Cádiz CF | 4:5pen. |
| 7/01/21 19:00 | SD Amorebieta | Real Sporting de Gijón | 0:1 |
| 7/01/21 19:00 | Deportiva Santa Eulalia del Rio | C. E. Sabadell F. C. | 2:0pen. |
| 7/01/21 19:00 | Girona F. C. | C. D. Lugo | 2:1t.pro. |
| 7/01/21 19:00 | Yeclano Deportivo | Valencia C. F. | 1:4 |
| 7/01/21 21:00 | Las Rozas CF | S. D. Eibar | 3:4t.pro. |
| 7/01/21 21:00 | CD Alcoyano | SD Huesca | 2:1 |
